We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution.It involves melting a cryo sample with a laser beam to AMINO COMPLEX allow dynamics of the embedded particles to occur.Once the laser beam is switched off, the sample revitrifies within just a few microseconds, t
Abstract Background Teleost paired fins are composed of two endoskeletal domains, proximal and distal radials, and an exoskeletal domain, the fin ray.The zebrafish pectoral fin displays elaborately patterned radials along the anteroposterior (AP) axis.Radials are considered homologous to tetrapod limb skeletons, and their patterning mechanisms in e
Cystic fibrosis (CF) is caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) protein.Thousands of CFTR mutations have been identified, but only a fraction are known to cause CF, with the most common being the prototypical class II CFTR mutation F508del.Elexacaftor-Tezacaftor-Ivacaftor (ETI) is a CFTR modulator that